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Objective: To investigate the expression of microRNA-12 99 (miR-1299) in esophageal carcinoma and its effects on the migration and invasion of esophageal cancer TE1 cells. Methods: The pathological tissue specimens and their matched paracancerous tissues of 58 patients with esophageal cancer were selected in the Fourth Hospital of Hebei Medical University from June 2017 to June 2018. The expression level of miR-1299 in cancer and corresponding adjacent tissues was detected by real-time fluorescent quantitative PCR. The relationship between miR-1299 expression and clinicopathological features was analyzed. After miR-1299-mimics were transfected into esophageal cancer TE1 cells for the overexpression of miR-1299, the proliferation of TE1 cells was detected by CCK-8 assay, and the migration and invasion abilities were detected by Transwell migration and invasion assays, respectively. Then the expressions of matrix metalloproteinase 11 (MMP11) and MMP16 mRNAs and proteins in miR-1299 over-expressed TE1 cells were detected by realtime fluorescent quantitative PCR and Western blotting, respectively. Results: The expression of miR-1299 in esophageal cancer tissues was significantly lower than that in the adjacent tissues (P 0.05), while the migration (P < 0.001) and invasion (P < 0.01) abilities decreased significantly, and the expressions of MMP11 and MMP16 mRNAs and proteins were obviously decreased (all P< 0.01). Conclusion: The expression of miR-1299 is down-regulated in esophageal cancer tissues and may inhibit the migration and invasion of esophageal cancer cells.
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Objective:To analyze the effect of levonorgestrel releasing intrauterine system ( Mirena) on endometrium in the pa-tients with anovulatory uterine bleeding. Methods:Totally 67 cases of anovulatory uterine hemorrhage were treated with Mirena. The menstrual blood volume, uterine endometrial thickness, hemoglobin, endometrial ET content and complications were recorded before and after the treatment. Results:After the 12-month treatment, the menstrual blood volume and endometrial thickness were significant-ly decreased, and hemoglobin was significantly increased (P<0. 01). The percentage of abnormal uterine bleeding was significantly decreased, the ET content in endometrium was significantly decreased (P<0.05), and the ET content was decreased significantly with the prolongation of the treatment time. The complication rate was reduced from 50. 74% to 7. 46%. Conclusion:Mirena is nota-bly effective in the treatment of the patients with anovulatory uterine bleeding.
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Objective To investigate the prevalence of vitiligo in China through a multi-center and larse-population epidemiological survey.Methods A community-based survey was conducted in 6 cities from 6 provinces.Cluster sampling method was used.Subjects were required to fulfill the self-report questionnaires and received physical examination by dermatologists.EpiData and SPSS11.5 were utilized for statistical analysis. Results Totally,19 974 patients participated in this study,and 17 345 valid questionnaires were retrieved with a return rate of 86.84%.Of them,122 were found to have vitiligo.The prevalence and standardized prevalence of vitiligo was 0.70% and 0.56% in all patients,0.95% (75) and 0.69% in male patients and 0.50% (47) and O.45% in female patients.respectively.A significant elevation was observed in the prevalence of vitiligo in males than in females (P<0.01).The prevalence of vitiligo was increased with age and peaked in patients aging from 60 to 69 years and those over 70 years.The age at onset of vitiligo varied from 0 to 19 years in 21.85% of these patients,from 20 to 49 years in 47.05%.The most connnon type was focal vitiligo,which accounted for 36.06%,while the rarest type wag segmental type (2.46%).The pesitivity rate of family history of vitiligo was 9.84% in patients and 1.31% in community population (P<0.01).About 31.97% of the patients complained of negative influence of vitiligo on quality of life.Conclusions The standardized prevalence of vitiligo is 0.56%in 6 provinces from China.Males seem to have a higher prevalence of vifiligo than females.
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Objective To investigate the therapeutic indications,dosage and prognosis of dermato-mucous membrane heamangioma(DMMH)in infants using low-dose superficial X-ray irradiation.Methods The patients were treated with a superficial X-ray machine(DERMOPAN typeⅡ)at voltages of29kV,43kV and50kV according to the depth and position of angioma,one or two times per week,1~2Gy per time,with the average total doses of5~10Gy and the maximal dose of20Gy.Results In a total of267patients,230cured,24markedly improved,10improved,and3failed.The cure rate was86.14%,and the total improve-ment rate was95.13%.Conclusion Treatment of DMMH with low-dose superfical X-ray is simple,conve-nient,safe,painless and cheap.The method has a high cure rate and is well accepted by the chlidren and their parents.
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Objective To approach the value of CT double windows technique in the location of lung X-ray knife.Methods The varying window width(W),window center(C) of peripheral type carcinoma of lung and their error were deltermined.The best double window was chosen and the clinical matherial was analysed.Results (1)The biggest error among different widow widths measuring foci was 7 mm.(2)The best double windows in the location of lung X-ray knife were W1 120~180,C1-700,W2 350,C2 15.(3)34 cases of lung carcinoma were observed with double windows,the outline of foci was contiuous and clear in the 15 cases of peripheral type carcinoma of lung and 8 cases of central type carcinoma of lung without hilum infiltration(67.6%),the inner outline of foci was disappear but could be remedied by mediastinum window in 11 cases of central type carcinoma of lung with hilum infiltration. Conclusion The double widows technique is provied with accuracy and without lack fidelity,and could reflect more condition of peritumour infiltration,it is clear superior to single display technique.